Frataxin antibody (AA 20-210)

Catalog No. ABIN7825383

This Rabbit Polyclonal antibody specifically detects Frataxin in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Frataxin antibody (AA 20-210) (ABIN7825383)

Target: Frataxin (FXN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Frataxin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Frataxin Antibody

Binding Specificity: AA 20-210

Purpose: Anti-FXN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FXN Antibody catalog # A00842-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FXN recombinant protein (Position: Q20-A210). Human FXN shares 79% and 71.4% amino acid (aa) sequence identity with mouse and rat FXN, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Frataxin

Target: Frataxin (FXN)

Alternative Name: FXN

Background: This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.

Molecular Weight: 14 kDa

Gene ID: 2395

UniProt: Q16595

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size