GCH1 antibody (AA 1-250)

Catalog No. ABIN7825429

The Rabbit Polyclonal anti-GCH1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect GCH1 in samples from Human, Mouse and Rat.

Quick Overview for GCH1 antibody (AA 1-250) (ABIN7825429)

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GCH1 Antibody

Binding Specificity: AA 1-250

Purpose: Anti-GCH1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GCH1 Antibody catalog # A01210-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GCH1 recombinant protein (Position: M1-S250). Human GCH1 shares 89.2% and 92.5% amino acid (aa) sequence identity with mouse and rat GCH1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GCH1

Background: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme.

Molecular Weight: 30 kDa

Gene ID: 2643

UniProt: P30793