HERC4 antibody (AA 18-1057)

Catalog No. ABIN7825447

The Rabbit Polyclonal anti-HERC4 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect HERC4 in samples from Human, Mouse and Rat.

Quick Overview for HERC4 antibody (AA 18-1057) (ABIN7825447)

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HERC4 Antibody

Binding Specificity: AA 18-1057

Purpose: Anti-HERC4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HERC4 Antibody catalog # A10089-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HERC4 recombinant protein (Position: D18-I1057). Human HERC4 shares 96.8% and 97% amino acid (aa) sequence identity with mouse and rat HERC4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HERC4

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Alternative Name: HERC4

Background: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins.

Molecular Weight: 107 kDa

Gene ID: 26091