HERC4 antibody (Middle Region)

Catalog No. ABIN2774773

The Rabbit Polyclonal anti-HERC4 antibody (ABIN2774773) specifically detects HERC4 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae samples.

Quick Overview for HERC4 antibody (Middle Region) (ABIN2774773)

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HERC4 Antibody

Binding Specificity: Middle Region

Purpose: HERC4 Antibody - middle region

Sequence: LVIQSTGGGE EYLPVSHTCF NLLDLPKYTE KETLRSKLIQ AIDHNEGFSL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Yeast: 83%

Characteristics: This is a rabbit polyclonal antibody against HERC4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HERC4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43298-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HERC4

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Alternative Name: HERC4

Background:

Background Information: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transf

Gene Name: HECT and RLD domain containing E3 ubiquitin protein ligase 4

Alternative Symbols: DKFZP564G092, KIAA1593

Protein Name: Probable E3 ubiquitin-protein ligase HERC4

Molecular Weight: 118kDa

Gene ID: 26091

NCBI Accession: NP_071362

UniProt: Q5GLZ8