IFT20 antibody (AA 1-132)

Catalog No. ABIN7825488

This Rabbit Polyclonal antibody specifically detects IFT20 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IFT20 antibody (AA 1-132) (ABIN7825488)

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-IFT20 Antibody

Binding Specificity: AA 1-132

Purpose: Anti-IFT20 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IFT20 Antibody catalog # A07253. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IFT20 recombinant protein (Position: M1-K132). Human IFT20 shares 98.5% amino acid (aa) sequence identity with mouse IFT20.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IFT20

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Alternative Name: IFT20

Background: This gene encodes a intraflagellar transport protein important for intracellular transport. The encoded protein forms part of a complex involved in trafficking of proteins from the Golgi body, including recycling of immune signalling components (Finetti et al., PubMed: 19855387). This gene is part of a complex set of sense-antisense loci that may be co-regulated. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 14.

Molecular Weight: 15 kDa

Gene ID: 90410

Pathways: Hedgehog Signaling