KPNB1 antibody (AA 8-871)

Catalog No. ABIN7825492

The Rabbit Polyclonal anti-KPNB1 antibody has been validated for WB. It is suitable to detect KPNB1 in samples from Zebrafish (Danio rerio).

Quick Overview for KPNB1 antibody (AA 8-871) (ABIN7825492)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KPNB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KPNB1 Antibody

Binding Specificity: AA 8-871

Purpose: Anti-Zebrafish Importin Beta/KPNB1 Antibody

Characteristics: Anti-Zebrafish Importin Beta/KPNB1 Antibody catalog #AZA0A0R4IMZ8. Tested in WB applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish Importin Beta/KPNB1 recombinant protein (Position: E8-K871)

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Alternative Name: KPNB1

Background: Importin subunit beta-1 is a protein that in humans is encoded by the KPNB1 gene. Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.

UniProt: A0A0R48

Pathways: Protein targeting to Nucleus