KIF3A antibody (AA 486-701)

Catalog No. ABIN7825495

The Rabbit Polyclonal anti-KIF3A antibody has been validated for WB. It is suitable to detect KIF3A in samples from Zebrafish (Danio rerio).

Quick Overview for KIF3A antibody (AA 486-701) (ABIN7825495)

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIF3A Antibody

Binding Specificity: AA 486-701

Purpose: Anti-Zebrafish KIF3A Antibody

Characteristics: Anti-Zebrafish KIF3A Antibody catalog # AZE9QB71. Tested in WB applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish KIF3A recombinant protein (Position: D486-Q701).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KIF3A

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Alternative Name: KIF3A

Background: Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the plus ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

Pathways: Hedgehog Signaling