MADD antibody (AA 4-335)

Catalog No. ABIN7825519

The Rabbit Polyclonal anti-MADD antibody has been validated for WB. It is suitable to detect MADD in samples from Zebrafish (Danio rerio).

Quick Overview for MADD antibody (AA 4-335) (ABIN7825519)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MADD Antibody

Binding Specificity: AA 4-335

Purpose: Anti-Zebrafish MADD Antibody

Characteristics: Anti-Zebrafish MADD Antibody catalog #AZA0A8M3AUB1. Tested in WB applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish MADD recombinant protein (Position: K4-Y335)

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: MADD

Background: MAP kinase-activating death domain protein is an enzyme that in humans is encoded by the MADD gene. Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.

UniProt: A0A8M31

Pathways: Caspase Cascade in Apoptosis