MPL antibody (AA 26-595)

Catalog No. ABIN7825562

This anti-MPL antibody is a Rabbit Polyclonal antibody detecting MPL in WB, FACS and ELISA. Suitable for Human.

Quick Overview for MPL antibody (AA 26-595) (ABIN7825562)

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-MPL Antibody

Binding Specificity: AA 26-595

Purpose: Anti-TPOR/MPL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TPOR/MPL Antibody catalog # A00483-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TPOR/MPL recombinant protein (Position: Q26-Q595). Human TPOR/MPL shares 79.6% amino acid (aa) sequence identity with mouse TPOR/MPL.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MPL

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Alternative Name: MPL

Background: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Molecular Weight: 90 kDa

Gene ID: 352

UniProt: P40238

Pathways: JAK-STAT Signaling