NFU1 antibody (AA 42-254)

Catalog No. ABIN7825605

This Rabbit Polyclonal antibody specifically detects NFU1 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFU1 antibody (AA 42-254) (ABIN7825605)

Target: NFU1 (NFU1 Iron-Sulfur Cluster Scaffold (NFU1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFU1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NFU1 Antibody

Binding Specificity: AA 42-254

Purpose: Anti-NFU1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NFU1 Antibody catalog # A06191-1. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NFU1 recombinant protein (Position: Q42-P254). Human NFU1 shares 93% amino acid (aa) sequence identity with mouse NFU1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NFU1

Target: NFU1 (NFU1 Iron-Sulfur Cluster Scaffold (NFU1))

Alternative Name: NFU1

Background: This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 28 kDa

Gene ID: 27247