Nidogen 1 antibody (AA 31-1209)

Catalog No. ABIN7825609

The Rabbit Polyclonal anti-Nidogen 1 antibody has been validated for ELISA, WB, IHC, IF and FACS. It is suitable to detect Nidogen 1 in samples from Mouse and Human.

Quick Overview for Nidogen 1 antibody (AA 31-1209) (ABIN7825609)

Target: Nidogen 1 (NID1)

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nidogen 1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nidogen 1 Antibody

Binding Specificity: AA 31-1209

Purpose: Anti-Entactin/NID1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Entactin/NID1 Antibody catalog # A05621. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Entactin/NID1 recombinant protein (Position: R31-H1209). Human Entactin/NID1 shares 85.6% amino acid (aa) sequence identity with mouse Entactin/NID1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Nidogen 1

Target: Nidogen 1 (NID1)

Alternative Name: NID1

Background: This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.

Molecular Weight: 150 kDa

Gene ID: 4811

UniProt: P14543