Anti-NUP85 Antibody catalog # A07147-1. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
Purification
Immunogen affinity purified.
Immunogen
E.coli-derived human NUP85 recombinant protein (Position: R84-S656). Human NUP85 shares 92.3% and 93.2% amino acid (aa) sequence identity with mouse and rat NUP85, respectively.
NUP85
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Target
NUP85
(Nucleoporin 85kDa (NUP85))
Alternative Name
NUP85
Background
This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants.