SEC63 antibody (AA 37-760)

Catalog No. ABIN7825792

The Rabbit Polyclonal anti-SEC63 antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect SEC63 in samples from Human, Mouse and Rat.

Quick Overview for SEC63 antibody (AA 37-760) (ABIN7825792)

Target: SEC63 (SEC63 Homolog (SEC63))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC63 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SEC63 Antibody

Binding Specificity: AA 37-760

Purpose: Anti-SEC63 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SEC63 Antibody catalog # A04852-1. Tested in WB, IHC, IF, ICC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SEC63 recombinant protein (Position: R37-D760). Human SEC63 shares 96.1% amino acid (aa) sequence identity with mouse SEC63.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse
Immunofluorescence, 5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SEC63

Target: SEC63 (SEC63 Homolog (SEC63))

Alternative Name: SEC63

Background: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.

Molecular Weight: 90 kDa

Gene ID: 11231

Pathways: ER-Nucleus Signaling