Syntaxin 4 antibody (AA 1-278)

Catalog No. ABIN7825906

The Rabbit Polyclonal anti-Syntaxin 4 antibody has been validated for WB, ELISA, IP, IF, IHC, ICC and FACS. It is suitable to detect Syntaxin 4 in samples from Human, Rat and Mouse.

Quick Overview for Syntaxin 4 antibody (AA 1-278) (ABIN7825906)

Target: Syntaxin 4 (STX4)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 1-278

Purpose: Anti-Syntaxin 4/STX4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Syntaxin 4/STX4 Antibody catalog # A05345-1. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Syntaxin 4/STX4 recombinant protein (Position: M1-I278). Human Syntaxin 4/STX4 shares 91.7% and 91.4% amino acid (aa) sequence identity with mouse and rat Syntaxin 4/STX4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: STX4

Background: Enables sphingomyelin phosphodiesterase activator activity. Involved in several processes, including cornified envelope assembly, positive regulation of immune effector process, and positive regulation of protein localization. Located in several cellular components, including basolateral plasma membrane, cytoplasmic vesicle, and lamellipodium. Part of SNARE complex. Is active in glutamatergic synapse and postsynapse. Implicated in autosomal recessive nonsyndromic deafness.

Molecular Weight: 37 kDa

Gene ID: 6810

UniProt: Q12846

Pathways: Synaptic Vesicle Exocytosis