UBE2F antibody (AA 1-185)

Catalog No. ABIN7826020

The Rabbit Polyclonal anti-UBE2F antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect UBE2F in samples from Human, Mouse and Rat.

Quick Overview for UBE2F antibody (AA 1-185) (ABIN7826020)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-UBE2F Antibody

Binding Specificity: AA 1-185

Purpose: Anti-UBE2F Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-UBE2F Antibody catalog # A12847-1. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBE2F recombinant protein (Position: M1-R185). Human UBE2F shares 96.8% and 96.2% amino acid (aa) sequence identity with mouse and rat UBE2F, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: Enables NEDD8 conjugating enzyme activity. Involved in protein neddylation. Predicted to be active in cytosol and nucleus.

Molecular Weight: 21 kDa

Gene ID: 140739

UniProt: Q969M7