Recombinant NFIX antibody (Center)

Catalog No. ABIN7826100

This anti-NFIX antibody is a Rabbit Monoclonal antibody detecting NFIX in IHC. Suitable for Human.

Quick Overview for Recombinant NFIX antibody (Center) (ABIN7826100)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV329

Product Details anti-NFIX Antibody

Binding Specificity: Center

Purpose: NFIX antibody (HMV329)

Immunogen: Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Placenta: A moderate to strong nuclear NFIX staining should be seen in stromal cells while trophoblast cells should remain NFIX negative.

Product Description/Background: NFIX antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Placenta: Trophoblast cells should remain NFIX negative while a moderate to strong nuclear NFIX staining should be seen in stromal cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NFIX

UniProt: Q14938

Pathways: Skeletal Muscle Fiber Development