Recombinant L1CAM antibody (AA 550-900)

Catalog No. ABIN7826108

The Mouse Monoclonal anti-L1CAM antibody has been validated for IHC. It is suitable to detect L1CAM in samples from Human.

Quick Overview for Recombinant L1CAM antibody (AA 550-900) (ABIN7826108)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 171R

Product Details anti-L1CAM Antibody

Binding Specificity: AA 550-900

Purpose: CD171 antibody (171R)

Immunogen: Recombinant fragment (around aa550-900) of human L1CAM protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: A strong membranous L1CAM staining should be seen in a subset of collecting ducts while the vast majority of tubuli remains L1CAM negative.

Product Description/Background: CD171 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Kidney: L1CAM staining must be absent in the vast majority of tubuli (L1CAM staining is limited to collecting ducts).

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: CD171

Background: Gene Alias: CAML1, Neural cell adhesion molecule L1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve- growth factor-inducible large external glycoprotein, NILE, S10, SPG1, Neural cell adhesion molecule L1

UniProt: P32004

Pathways: Synaptic Membrane