Recombinant Claudin 3 antibody (Intracellular)

Catalog No. ABIN7826112

This anti-Claudin 3 antibody is a Rabbit Monoclonal antibody detecting Claudin 3 in IHC. Suitable for Human.

Quick Overview for Recombinant Claudin 3 antibody (Intracellular) (ABIN7826112)

Target: Claudin 3 (CLDN3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Claudin 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV309

Product Details anti-Claudin 3 Antibody

Binding Specificity: Intracellular

Purpose: Claudin 3 antibody (HMV309)

Immunogen: Synthetic peptide encompassing a sequence within the Intracellular domain of human Claudin 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A strong membranous CLDN3 staining should be seen in bile ducts. In addition, a weak to moderate membranous CLDN3 staining should be seen in hepatocytes (often seen predominantly at the bile secreting pole).

Product Description/Background: Claudin 3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Stomach: Most epithelial cells should be CLDN3 negative, while a strong membranous CLDN3 staining of a small subset of epithelial cells in the neck and in glandular pits must be seen.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for Claudin 3

Target: Claudin 3 (CLDN3)

Alternative Name: Claudin 3

Background: Gene Alias: claudin 3,C7orf1,CPE-R2,CPETR2,HRVP1,RVP1

UniProt: O15551

Pathways: Hepatitis C