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Recombinant GATA3 antibody

This anti-GATA3 antibody is a Rabbit Monoclonal antibody detecting GATA3 in IHC. Suitable for Human.
Catalog No. ABIN7826118

Quick Overview for Recombinant GATA3 antibody (ABIN7826118)

Target

See all GATA3 Antibodies
GATA3 (GATA Binding Protein 3 (GATA3))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This GATA3 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

550R
  • Purpose

    GATA3 antibody (550R)

    Immunogen

    Recombinant human Gata3 protein

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.

    Product Description/Background: GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    Expiry Date

    24 months
  • Target

    GATA3 (GATA Binding Protein 3 (GATA3))

    Alternative Name

    GATA3

    Background

    Gene Alias: GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3

    UniProt

    P23771

    Pathways

    Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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