Histone 3 antibody
Quick Overview for Histone 3 antibody (ABIN7826121)
Target
See all Histone 3 (H3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Histone H3 / H3-3A antibody (903M)
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Immunogen
- Recombinant human H3.3 histone A fragment
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Lymph node: A strong nuclear Histone H3 staining should be seen in all cells while strongest staining intensity occurs in cells of germinal centres.
Product Description/Background: Histone H3 / H3-3A antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon, Kidney: Histone H3 staining should be seen in nuclei only. Cytoplasmic staining should not be seen.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- Histone 3 (H3) (Histone H3 (H3))
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Alternative Name
- Histone H3
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Background
- Gene Alias: H3.3 Histone A, H3.3A, H3F3A, H3F3, H3 Histone Family Member 3A, H3 Histone, Family 3A, Histone H3.3, BRYLIB1, H3- 3B, H3.3B, H3F3B
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UniProt
- P84243
Target
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