Recombinant T-Box 1 antibody

Catalog No. ABIN7826147

The Rabbit Monoclonal anti-T-Box 1 antibody has been validated for IHC. It is suitable to detect T-Box 1 in samples from Human.

Quick Overview for Recombinant T-Box 1 antibody (ABIN7826147)

Target: T-Box 1 (TBX1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This T-Box 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV321

Product Details anti-T-Box 1 Antibody

Purpose: Brachyury antibody (HMV321)

Immunogen: Recombinant human Brachyury fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Chordoma with previously demonstrated brachyury expression.

Product Description/Background: Brachyury antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Normal kidney: Brachyury staining must be completely absent in all cells (any other normal tissue could be used instead).

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for T-Box 1

Target: T-Box 1 (TBX1)

Alternative Name: Brachyury

Background: Gene Alias: T-box transcription factor T,SAVA,T,TFT

UniProt: O15178

Pathways: Retinoic Acid Receptor Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation