Recombinant ACLY antibody

Catalog No. ABIN7828029

The Rabbit Monoclonal anti-ACLY antibody (Clone A772) (ABIN7828029) specifically detects ACLY in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant ACLY antibody (ABIN7828029)

Target: ACLY (ATP Citrate Lyase (ACLY))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ACLY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A772

Product Details anti-ACLY Antibody

Purpose: Recombinant ATP Citrate Lyase Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ATP Citrate Lyase

Background: OTTHUMP,ACLY,ACL,ATPCL,CLATP,ATP citrate (pro-S) lyase,ATP citrate lyase,ATP citrate synthase,ATP-citrate (pro-S-)-lyase,ATPcitrate synthase,ATP-citrate synthase,Citrate cleavage enzyme,OTTHUMP00000164773,ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the keystep for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis. Nutrients and hormones regulate theexpression level and phosphorylation of ATP-citrate lyase. It is phosphorylated by GSK-3 on Thr446 and Ser450. Ser455 of ATP-citrate lyasehas been reported to be phosphorylated by PKA and Akt. Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrateand enhances the catalytic activity of the enzyme. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 120 kDa

Observed MW: 125 kDa The actual band is not consistent with the expectation.

UniProt: P53396

Pathways: Warburg Effect