Recombinant CD3D antibody

Catalog No. ABIN7828082

The Rabbit Monoclonal anti-CD3D antibody is suitable to detect CD3D in samples from Human. It has been validated for WB, IHC, IP, FACS and IF.

Quick Overview for Recombinant CD3D antibody (ABIN7828082)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: A525

Product Details anti-CD3D Antibody

Purpose: Recombinant CD3D Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human CD3D fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:2000-1:10000,IHC 1:200-1:1000,IF 1:50-1:200,FCM 1:50-1:100,IP 1:25-1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background: IMD,CD3D,CD3-DELTA,IMD19,T3D,T-Cell Receptor T3 Delta Chain,T-cell surface glycoprotein CD3 delta chain,T3,CD3 delta,CD3D is a component of the TCR-CD3 complex, which is present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition, CD3D plays an essential role in thymocyte differentiation. It participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. It interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 19 kDa

Observed MW: 23 kDa The actual band is not consistent with the expectation.

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events