Recombinant eEF1A1 antibody

Catalog No. ABIN7828184

The Rabbit Monoclonal anti-eEF1A1 antibody is suitable to detect eEF1A1 in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for Recombinant eEF1A1 antibody (ABIN7828184)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: A521

Product Details anti-eEF1A1 Antibody

Purpose: Recombinant eEF1A1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human eEF1A1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IF 1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: eEF1A1

Background: EEF,PTI,EE1A,HNGC,LENG,CCS,EEF1A1,CCS-3,CCS3,EE1A1,EEF-1,EEF1A,EF-Tu,EF1A,GRAF-1EF,HNGC:16303,LENG7,PTI1,eEF1A-1,eEF1A, also named elongation factor Tu (EF-Tu), is one of the eukaryotic elongation factors (eEFs), that function in translation. eEF1A binds GTP and interacts with amino acyl-tRNAs to promote the recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 50 kDa

Observed MW: 55 kDa The actual band is not consistent with the expectation.

UniProt: P68104