Recombinant Histone H2A antibody (H2AK5ac)

Catalog No. ABIN7828262

The Rabbit Monoclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Rat and Mouse. It has been validated for WB and ChIP.

Quick Overview for Recombinant Histone H2A antibody (H2AK5ac) (ABIN7828262)

Target: Histone H2A

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: A109

Product Details anti-Histone H2A Antibody

Binding Specificity: H2AK5ac

Purpose: Recombinant Histone H2A (Acetyl Lys5) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Acetylated human histone H2A (Lys5) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000-1:2000,ChIP 6 μg/ 5x106 cells

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Histone H2A

Target: Histone H2A

Background: H2AFM,HIST1H2AB,H2AFA,HIST1H2AE,H2AC4,H2AC8,Histone post-translational modifications (PTMs), known as the ""histone code"", are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5, 12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 14 kDa

Observed MW: 14 kDa

UniProt: P04908