Recombinant Histone H4 antibody (H4K12me1)

Catalog No. ABIN7828285

This Rabbit Monoclonal antibody specifically detects Histone H4 in WB, IHC, IF, IP, ChIP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant Histone H4 antibody (H4K12me1) (ABIN7828285)

Target: Histone H4

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS)

Clone: A838

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K12me1

Purpose: Recombinant Histone H4 (Mono Methyl Lys12) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Monomethylated human histone H4 (Lys12) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:100-1:500,IF 1:50-1:200,FCM 1:50,ChIP 6 μg/5x106 cells,IP 1:25-1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4

Background: H4F,methyl histone H,methylated histone H,HIST2H,HIST1H,HISTH4H,histone H,HIST2H4B,H4/o,histone H4,HIST1H4A,FO108,H4/p,HIST1H4,Hist4h4,HISTH4H4,methyl histone H4,methylated histone H4,H4/A,H4FA,H4/I,H4FI,HIST1H4B,H4/G,H4FG,HIST1H4C,H4/B,H4FB,HIST1H4D,H4/J,H4FJ,HIST1H4E,H4/C,H4FC,HIST1H4F,H4/H,H4FH,HIST1H4H,H4/M,H4FM,HIST1H4I,H4/E,H4FE,H4/D,H4FD,HIST1H4K,H4/K,H4FK,HIST1H4L,H4/N,H4F2,H4FN,HIST2H4,HIST2H4A,H4,H4C1,H4C2,H4C3,H4C4,H4C5,H4C6,H4C8,H4C9,H4C11,H4C12,H4C13,H4C14,H4C15,H4-16,A,I,H4B,H4J,H4H,H4M,H4E,HIST1H4J,H4D,H4C,H4/p,histone H4,HIST1H4B H4/I,The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as ""histone code"" or ""epigenetic code"". Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys5, 8, 12, 20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methyltransferases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 11 kDa

Observed MW: 11 kDa

UniProt: P62805