Recombinant SET7 antibody

Catalog No. ABIN7828560

The Rabbit Monoclonal anti-SET7 antibody (Clone A733) (ABIN7828560) specifically detects SET7 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant SET7 antibody (ABIN7828560)

Target: SET7 (SETD8P1) (SET Domain Containing 8 Pseudogene 1 (SETD8P1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SET7 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A733

Product Details anti-SET7 Antibody

Purpose: Recombinant SET7 Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for SET7

Target: SET7 (SETD8P1) (SET Domain Containing 8 Pseudogene 1 (SETD8P1))

Alternative Name: SET7

Background: KMT,SETD,KIAA,SET,SETD7,KMT7,SET7,SET7/9,SET9,KIAA1717,SET7/SET9 is a member of the SET domain-containing family, and can specifically methylate Lys4 on histone H3. Like most other lysine directed histone methyltransferases, it contains a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. Methylation of histone H3 Lys4 enhances transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes. In addition, methylation of lysine 4 blocks transcriptional repression by inhibiting the binding of the NURD histone deacetylation complex to the amino-terminal tail of histone H3 and interfering with SUV39H1 mediated methylation of histone H3 Lys9. SET7/SET9 is highly active on free histone H3, but only very weakly methylates H3 within nucleosomes. Besides histones, SET7/SET9 also methylates Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein. Methylation of TAF10 stimulates transcription in a promoter-specific manner by increasing the affinity of TAF10 for RNA polymerase II, which may potentiate pre-initiation complex formation. Methylation of p53 at Lys372 increases protein stability and leads to upregulation of target genes such as p21. Thus the loss of SET7/SET9 may represent another mechanism for the inactivation of p53 in human cancers. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 41 kDa

Observed MW: 47 kDa The actual band is not consistent with the expectation.

UniProt: Q8WTS6