Recombinant Topoisomerase I antibody

Catalog No. ABIN7828628

The Rabbit Monoclonal anti-Topoisomerase I antibody is suitable to detect Topoisomerase I in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, IP and FACS.

Quick Overview for Recombinant Topoisomerase I antibody (ABIN7828628)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: A475

Product Details anti-Topoisomerase I Antibody

Purpose: Recombinant Topoisomerase I Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human Topoisomerase I fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:100-1:500,IF 1:50,FCM 1:50-1:100,IP 1:25-1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topoisomerase I

Background: TOP,TOP1,TOPI,DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 91 kDa

Observed MW: 108 kDa The actual band is not consistent with the expectation.

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance