beta Actin antibody (N-Term)

Catalog No. ABIN7833123

This Mouse Monoclonal antibody specifically detects beta Actin in WB, ELISA, IHC and DB. It exhibits reactivity toward Human, Mouse, Rat, Chicken and Rabbit.

Quick Overview for beta Actin antibody (N-Term) (ABIN7833123)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Purpose: Anti-Beta-Actin, (animal), mouse monoclonal

Cross-Reactivity (Details): Chicken, human, mouse, rabbit, rat
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Immunogen: Synthetic peptide derived from the N-terminal of the human beta-actin

Isotype: IgG2b

Application Details

Application Notes: 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: IgG2b purified on Protein A n 10 mM PBS, pH 7.2, with 1 % BSA and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks

Expiry Date: 36 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Beta-Actin

Background:

Background: Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.

Additional information: 10 mM PBS, pH 7,2, with 1 % BSA and 0,05 % sodium azide

Molecular Weight: 42 kDa

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction