CO antibody

Catalog No. ABIN7833193

The Chicken Polyclonal anti-CO antibody is suitable to detect CO in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for CO antibody (ABIN7833193)

Target: CO

Reactivity: Arabidopsis thaliana

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-CO | Constans (chicken antibody)

Cross-Reactivity (Details): Arabidopsis thaliana (recombinant CO)
Not reactive in: Brassica oleracea var. oleracea, Brassica rapa, Raphanus sativus, Vitis vinifera

Predicted Reactivity: Camelina sativa,Chrysanthemum sp.,Solanum lycopersicum

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein

Application Details

Application Notes: 1: 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: for reconstitution please add 50 μL of dest, water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: CO

Background:

Background: CO (CONSTANS) is a putative transcription factor which is involved in the long day flowering pathway and has been implied to control mediation between circadian clock and flowering. It regulates flowering time by interactions with SOC1 (suppressor of CO 1), TF1 (terminal flower 1), and FT (flowering locus T). Furthermore it is involved in proline and ethylene biosynthesis.

Additional information: Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.

Molecular Weight: 41.9 kDa

Gene ID: 831441

UniProt: Q39057