FHL5 antibody

Catalog No. ABIN7833256

The Mouse Monoclonal anti-FHL5 antibody is suitable to detect FHL5 in samples from Alfalfa, Arabidopsis thaliana and Zea mays. It has been validated for WB and IF.

Quick Overview for FHL5 antibody (ABIN7833256)

Target: FHL5 (Four and A Half LIM Domains 5 (FHL5))

Reactivity: Alfalfa, Arabidopsis thaliana, Zea mays

Host: Mouse

Clonality: Monoclonal

Conjugate: This FHL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 14H4G8

Product Details anti-FHL5 Antibody

Purpose: Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)

Cross-Reactivity (Details): Arabidopsis thaliana, Medicago sativa, Zea mays
Not reactive in: Chlamydomonas reinhardtii

Predicted Reactivity: Agropyron cristatum,Beta vulgaris,Betula luminifera,Brassica napus,Brassica rapa subsp. pekinensis,Capsella rubella,Castanea sativa,Chorispora bungeana,Cyanidioschyzon merolae strain 10D,Glycine max,Glycine soja,Halogeton glomeratus,Medicago truncatula,Malus domestica,Oryza sativa,Pisum sativun,Solanum lysopersicum,Solanum tuberosum,Phaseolus vulgaris,Picea abies,Picea sitchensis,Prunus avium,Ricinus communis,Rubus plicatus,Theobroma cacao,Triticum aestivum,Zea mays,Vicia faba

Purification: Immunogen affinity purified serum

Immunogen: Around 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620

Isotype: IgG1

Application Details

Application Notes: 1: 500 (IF), 1 : 1000 1: 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL, of sterile or deionized water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for FHL5

Target: FHL5 (Four and A Half LIM Domains 5 (FHL5))

Alternative Name: ACT

Background:

Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Additional information: This antibody was produced using CHO cells expression system.

Molecular Weight: 41.6 | 45 kDa

Gene ID: 818339

UniProt: P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497