SPA antibody (Agarose Beads)
Quick Overview for SPA antibody (Agarose Beads) (ABIN7833390)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Purpose
- Anti-Staphylococcal Protein A, agarose-conjugated
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Cross-Reactivity (Details)
- Protein A from Staphylococcus aureus
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Purification
- Immunogen affinity purified
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Immunogen
- Recombinant Protein A
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Immunogen affinity purified in PBS pH 7.2. Contains 0.075 % sodium azide, coupled to immunoprecipiation gel.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Do not freeze
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at +4-8°C. Do not freeze.
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- SPA (Immunoglobulin G Binding Protein A (SPA))
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Alternative Name
- Staphylococcal Protein A
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Background
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Background: Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Additional information: Antibodies were isolated from immune eggs, affinity purified on a protein A column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads, Beads were washed extensively with after the blocking of the residual NHS sites
Target
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