SPA antibody (FITC)

Catalog No. ABIN7833391

The Chicken Polyclonal anti-SPA antibody is suitable to detect SPA in samples from Staphylococcus aureus. It has been validated for IHC.

Quick Overview for SPA antibody (FITC) (ABIN7833391)

Target: SPA (Immunoglobulin G Binding Protein A (SPA))

Reactivity: Staphylococcus aureus

Host: Chicken

Clonality: Polyclonal

Conjugate: This SPA antibody is conjugated to FITC

Application: Immunohistochemistry (IHC)

Product Details anti-SPA Antibody (FITC)

Purpose: Anti-Staphylococcal Protein A, FITC-conjugated

Cross-Reactivity (Details): Staphylococcus aureus

Purification: Immunogen affinity purified

Immunogen: Recombinant Protein A

Application Details

Application Notes: To be determined by the end user

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified in PBS pH 7.2. Contains 0.075 % sodium azide, coupled to immunoprecipiation gel.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for SPA

Target: SPA (Immunoglobulin G Binding Protein A (SPA))

Alternative Name: Staphylococcal Protein A

Background:

Background: Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Additional information: Antibodies were isolated from immune eggs, affinity purified on a Protein A column and conjugated to fluoroscein isothiocyanate (FITC).