NAB1 antibody

Catalog No. ABIN7833436

The Rabbit Polyclonal anti-NAB1 antibody is suitable to detect NAB1 in samples from Chlamydomonas reinhardtii. It has been validated for WB.

Quick Overview for NAB1 antibody (ABIN7833436)

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAB1 Antibody

Purpose: Anti-NAB1 | nucleic acid binding protein 1, Chlamydomonas

Cross-Reactivity (Details): Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana CSP1- CSP4.

Purification: Antigen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii NAB1 protein sequence

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL of sterile or deionized water.

Buffer: Antigen affinity purified serum, in PBS pH 7.4

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for NAB1

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Alternative Name: NAB1

Background:

Background: In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).

Additional information: The NAB1 gene ID in Phytozome is Cre06.g268600_45321. There are two transcipts:Cre06.g268600.4532.1 - - 35,324 kDa and Cre06.g268600.4532.2 - - 26,539 kDa. In cell bodies of gametes, produce proteins from both transcripts, while in vegetative cells only one transcript is translated to NAB1 protein (26.5 kDa).

Molecular Weight: 26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)

UniProt: Q8GV23