PIF4 antibody

Catalog No. ABIN7833465

This Goat Polyclonal antibody specifically detects PIF4 in WB. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for PIF4 antibody (ABIN7833465)

Target: PIF4

Reactivity: Arabidopsis thaliana

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Solanum lycopersicum, Vitis vinifera

Predicted Reactivity: Brassica napus,Brassica rapa,Camelina sativa,Eutrema salsugineum

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PIF4

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: PIF4

Background:

Background: PIF4 (PHYTOCHROME INTERACTING FACTOR 4) is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.

Additional information: PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light). Extraction of PIF proteins is described in Shen et al. (2007).

Molecular Weight: 48.3 | 60 kDa

Gene ID: 818903

UniProt: Q8W2F3