RNF112 antibody

Catalog No. ABIN7833520

The Mouse Monoclonal anti-RNF112 antibody is suitable to detect RNF112 in samples from Aequorea victoria. It has been validated for WB and ELISA.

Quick Overview for RNF112 antibody (ABIN7833520)

Target: RNF112 (Ring Finger Protein 112 (RNF112))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNF112 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF112 Antibody

Purpose: Anti-BFP, GFP, YFP | Fluorescent Protein, clone 3A6

Cross-Reactivity (Details): BFP, GFP, YFP

Purification: Protein A purified

Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717

Isotype: IgG1

Application Details

Application Notes: 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recommended antibody concentration: 0.5 mg/mL (when dissolved at 0.5 mg/mL, the BSA concentration will be 1 % ). Recommended solvent, 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05 %) is recommended for long term storage. •For a 0.5 mg/mL antibody concentration in 1 % BSA, dissolve in 200 μL buffer.

Buffer: Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store lyophilized/reconstituted at 2-8°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for RNF112

Target: RNF112 (Ring Finger Protein 112 (RNF112))

Alternative Name: BFP

Background: Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.