Arabinogalactan-Protein antibody

Catalog No. ABIN7833697

The Rat Monoclonal anti-Arabinogalactan-Protein antibody is suitable to detect Arabinogalactan-Protein in samples from Higher Plants and Fern (Asplenium). It has been validated for IF and ELISA.

Quick Overview for Arabinogalactan-Protein antibody (ABIN7833697)

Target: Arabinogalactan-Protein

Reactivity: Higher Plants, Fern (Asplenium)

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA

Clone: LM2

Product Details

Purpose: Anti-Arabinogalactan-protein, AGP (monoclonal, clone LM2)

Cross-Reactivity (Details): Higher plants, ferns and mosses
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Immunogen: Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa

Isotype: IgM

Application Details

Application Notes: 1:10 (ELISA, IF)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Cell culture supernatant.

Storage: 4 °C,-20 °C

Storage Comment: Store at +4°C (short term) and at -20°C (long term).

Target Details

Target: Arabinogalactan-Protein

Background:

Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.

Additional information: Contains 0.05 % Sodium Azide.This antibody is made to rice arabinogalactan-proteins (AGPs) and it recognizes a carbohydrate epitope containing B-linked glucuronic acid. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50 %) by 70 mg/mL 1-O-methyl-B-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-B-D-GlcA.