Fc Receptor, IgG, Low Affinity IV (FCGR4) antibody

Catalog No. ABIN7835824

The Armenian Hamster Monoclonal anti-Fc Receptor, IgG, Low Affinity IV antibody is suitable to detect Fc Receptor, IgG, Low Affinity IV in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for Fc Receptor, IgG, Low Affinity IV (FCGR4) antibody (ABIN7835824)

Target: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 9E9

Product Details

Purpose: Armenian Hamster monoclonal antibody to CD16.2

Specificity: Recognizes mouse CD16.2

Purification: The antibody was purified by affinity chromatography.

Immunogen: FCgammaR4 -EC domain fusion with IgG1 Fc

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details

Target: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Alternative Name: FCGR4

Background: Low affinity immunoglobulin gamma Fc region receptor IV, CD16-2, FcgammaRIV

Gene ID: 246256