CD48 antibody (PE)

Catalog No. ABIN7836403

The Mouse Monoclonal anti-CD48 antibody is suitable to detect CD48 in samples from Human. It has been validated for FACS and IF.

Quick Overview for CD48 antibody (PE) (ABIN7836403)

Target: CD48

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD48 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 156-4H9

Product Details anti-CD48 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD48

Specificity: Recognizes human CD48

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD48.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD48

Target: CD48

Alternative Name: CD48

Background: BCM1, BLAST1, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, SLAMF2, Signaling lymphocytic activation molecule 2, TCT.1, CD antigen CD48

Gene ID: 962

UniProt: P09326