ITGA6 antibody (PE)

Catalog No. ABIN7836427

The Rat Monoclonal anti-ITGA6 antibody is suitable to detect ITGA6 in samples from Human. It has been validated for FACS and IF.

Quick Overview for ITGA6 antibody (PE) (ABIN7836427)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4F10

Product Details anti-ITGA6 Antibody (PE)

Purpose: Rat monoclonal antibody PE labled to CD49f

Specificity: Recognizes human CD49f

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD49f.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: ITGA6

Background: Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD antigen CD49f)

Gene ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex