CD51 antibody (FITC)

Catalog No. ABIN7836446

The FITC-conjugated Mouse Monoclonal anti-CD51 antibody (Clone 13C2) (ABIN7836446) specifically detects CD51 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD51 antibody (FITC) (ABIN7836446)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 13C2

Product Details anti-CD51 Antibody (FITC)

Purpose: Mouse monoclonal antibody FITC labled to CD51

Specificity: Recognizes human CD51

Purification: The antibody was purified by affinity chromatography.

Immunogen: Cell suspension containing osteoclasts from osteoclastomas

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: ITGAV

Background: MSK8, VNRA, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51

Gene ID: 3685

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex