CD52 antibody (PE)

Catalog No. ABIN7836448

The Mouse Monoclonal anti-CD52 antibody is suitable to detect CD52 in samples from Human. It has been validated for FACS and IF.

Quick Overview for CD52 antibody (PE) (ABIN7836448)

Target: CD52

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD52 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HI186

Product Details anti-CD52 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD52

Specificity: Recognizes human CD52

Purification: The antibody was purified by affinity chromatography.

Immunogen: Human tonsil

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD52

Target: CD52

Alternative Name: CD52

Background: CDW52, HE5, CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5, CD52

Gene ID: 1043

UniProt: P31358