CD53 antibody (FITC)

Catalog No. ABIN7836450

This Mouse Monoclonal antibody specifically detects CD53 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD53 antibody (FITC) (ABIN7836450)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C53-2

Product Details anti-CD53 Antibody (FITC)

Purpose: Mouse monoclonal antibody FITC labled to CD53

Specificity: Recognizes human CD53

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD53.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD antigen CD53

Gene ID: 963

UniProt: P19397