L-Selectin antibody (Alexa Fluor 700)

Catalog No. ABIN7836510

This Rat Monoclonal antibody specifically detects L-Selectin in FACS and IF. It exhibits reactivity toward Mouse.

Quick Overview for L-Selectin antibody (Alexa Fluor 700) (ABIN7836510)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is conjugated to Alexa Fluor 700

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MEL-14

Product Details anti-L-Selectin Antibody (Alexa Fluor 700)

Purpose: Rat monoclonal antibody AF700 labled to CD62L

Specificity: Recognizes mouse CD62L

Purification: The antibody was purified by affinity chromatography.

Immunogen: C3H/eb mouse B lymphoma 38C-13

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: SELL

Background: LNHR, LYAM1, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, TQ1, gp90-MEL, CD62L

Gene ID: 20343