CD90 antibody (FITC)
Quick Overview for CD90 antibody (FITC) (ABIN7836681)
Target
See all CD90 (THY1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Rat monoclonal antibody FITC labled to CD90
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Specificity
- Recognizes mouse CD90
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Purification
- The antibody was purified by affinity chromatography.
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Immunogen
- Mouse T-cell hybridoma C6/G8
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anti-CD90 (THY1) (AA 17-132) antibody
THY1 Reactivity: Human WB, IHC, ICC, ELISA Host: Mouse Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) (AA 31-120) antibodyTHY1 Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB03970 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3F9 unconjugated
anti-CD90 (THY1) antibodyTHY1 Reactivity: Human, Monkey, Baboon FACS, IHC, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 Reactivity: Human, Pig, Horse, Non-Human Primate FACS Host: Mouse Monoclonal 5E10 FITC
anti-CD90 (THY1) antibodyTHY1 Reactivity: Mouse FACS, CyTox Host: Mouse Monoclonal F7D5 unconjugated
anti-CD90 (THY1) antibodyGMP Grade THY1 Reactivity: Human FACS Host: Mouse Monoclonal F15-42-1 unconjugated
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Application Notes
- Flow: 10 μL / assay
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Protocol
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Shipped and store at 4°C for one year. Do not freeze.
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- CD90 (THY1)
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Alternative Name
- THY1
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Background
- Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90
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Gene ID
- 21838
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Pathways
- Cell-Cell Junction Organization
Target
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