LY6 antibody (PE)

Catalog No. ABIN7840916

The PE-conjugated Rat Monoclonal anti-LY6 antibody (Clone D7) (ABIN7840916) specifically detects LY6 in FACS and IF. The antibody is reactive with Mouse samples.

Quick Overview for LY6 antibody (PE) (ABIN7840916)

Target: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LY6 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: D7

Product Details anti-LY6 Antibody (PE)

Purpose: Rat monoclonal antibody PE labled to Ly-6A/E

Specificity: Recognizes mouse Ly-6A/E

Purification: The antibody was purified by affinity chromatography.

Immunogen: IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for LY6

Target: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Alternative Name: Ly6

Background: Ly6, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly67, Sca-2, Tsa-1, Lymphocyte antigen 6E, Ly-6E, Stem cell antigen 2, Thymic shared antigen 1, TSA-1

Gene ID: 110454