CD163 antibody (PE-Cy5)

Catalog No. ABIN741582

This anti-CD163 antibody is a Rabbit Polyclonal antibody detecting CD163 in WB and FACS. Suitable for Human, Mouse, Rat, Pig, Dog and Cow.

Quick Overview for CD163 antibody (PE-Cy5) (ABIN741582)

Target: CD163

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD163 antibody is conjugated to PE-Cy5

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-CD163 Antibody (PE-Cy5)

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Predicted Reactivity: Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD163/M130

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
IHC-P(1:100-500)
IF(IHC-P)(1:100-500)
IF(ICC)(1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD163

Target: CD163

Alternative Name: CD163/M130

Background:

Synonyms: M13, MM13, Scavenger receptor cysteine-rich type 1 protein M13, Hemoglobin scavenger receptor, CD163, M130

Background: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH -dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Molecular Weight: 130kDa

Gene ID: 9332

UniProt: Q86VB7