SREBF2 antibody (Biotin)

Catalog No. ABIN741707

The Rabbit Polyclonal anti-SREBF2 antibody is suitable to detect SREBF2 in samples from Human, Rat, Mouse and Pig. It has been validated for WB and IHC (p).

Quick Overview for SREBF2 antibody (Biotin) (ABIN741707)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human, Rat, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SREBF2 Antibody (Biotin)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SREBP-2

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBP-2

Background:

Synonyms: Sterol Regulatory Element Binding Protein-2, MGC124823, Srebf2_retired, SREBP-2, SREBP2, SRBP2_HUMAN, Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, bHLHd2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2.

Background: Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. ab30682 detects both precursor and active forms of SREBP2 in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.

Molecular Weight: 126kDa

Gene ID: 6721

Pathways: Regulation of Lipid Metabolism by PPARalpha