ICOS antibody (AA 51-150) (HRP)

Catalog No. ABIN742389

The HRP-conjugated Rabbit Polyclonal anti-ICOS antibody (ABIN742389) specifically detects ICOS in WB, ELISA and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ICOS antibody (AA 51-150) (HRP) (ABIN742389)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICOS antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ICOS Antibody (HRP)

Binding Specificity: AA 51-150

Specificity: This antibody may also have a secondary cross-reactivity with AP-1 complex-associated regulatory protein due to a 77 % non-contiguous sequence similarity in the corresponding immunogen region.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ICOS

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS/CD278

Background:

Synonyms: AILIM, CD278, CVID1, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, ICOS

Background: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

Gene ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints