PDE4B antibody (AA 1-736)

Catalog No. ABIN7869543

The Rabbit Polyclonal anti-PDE4B antibody is suitable to detect PDE4B in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for PDE4B antibody (AA 1-736) (ABIN7869543)

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDE4B Antibody

Binding Specificity: AA 1-736

Purpose: PDE4B Antibody / Phosphodiesterase 4B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PDE4B recombinant protein (Position: M1-T736) was used as the immunogen for the PDE4B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDE4B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PDE4B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B

Background: PDE4B antibody detects Phosphodiesterase 4B, an enzyme that hydrolyzes cyclic AMP (cAMP) into AMP, terminating cAMP-dependent signaling. PDE4B belongs to the type 4 phosphodiesterase family, which provides fine control of intracellular cAMP concentrations. The PDE4B antibody is used in research focused on inflammation, neuroscience, and psychiatric disorders involving cAMP signaling dysregulation.

PDE4B is encoded by the PDE4B gene on human chromosome 1p31.3. The protein exists in multiple isoforms ranging from 70 to 100 kilodaltons, all containing a catalytic domain, upstream conserved regions (UCR1 and UCR2), and regulatory sequences that determine localization and activation. PDE4B localizes to cytoplasmic and perinuclear regions and interacts with signaling complexes via A-kinase anchoring proteins (AKAPs).

The PDE4B antibody identifies characteristic isoforms in western blot. Functionally, PDE4B hydrolyzes cAMP generated by G protein-coupled receptor activation, shaping responses to neurotransmitters, hormones, and immune stimuli. It regulates protein kinase A (PKA) activity and downstream transcription factors such as CREB.

In immune cells, PDE4B modulates cytokine production and inflammatory responses. Inhibition of PDE4B enhances cAMP signaling, reducing pro-inflammatory cytokines like TNF-alpha and IL-6. In the central nervous system, PDE4B regulates neuronal excitability, memory, and emotional processing. Genetic and pharmacologic studies implicate PDE4B dysfunction in depression, schizophrenia, and addiction.

PDE4B is a therapeutic target for anti-inflammatory and neuropsychiatric drugs. Selective inhibitors such as rolipram and roflumilast have been developed to modulate cAMP signaling. NSJ Bioreagents provides a validated PDE4B antibody optimized for its applications, supporting investigation into cyclic nucleotide metabolism, immune regulation, and neuronal signaling.

UniProt: Q07343

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction